Application of agricultural materials as ingredient in the medium for Cordyceps militaris production and development of Jelly Product from Spent Mushroom Substrate
The objective of this research was to study the effect of agricultural materials as ingredients in culture media for the fungus Cordyceps militaris on the growth and the active substance contents of Cordyceps militaris. The experiment was carried out in a Completely Randomized Design (CRD) using 3 varieties of rice (Sinlek, Mae Phaya Tongdam and Sangyod)
and 3 protein sources (egg, silk worm and pupa) as culture medias, totally 11 treatments with 3 replications. The Cordyceps militaris were conducted for 7 weeks at 20 degree Celsius. The fruiting body length, fruiting body number, fresh weight, dry weight, Cordycepin and Adenosine contents were recorded for data collection. The results showed that treatment number 8, which had a culture media comprising of 30 g of Sinlek rice and 350 g of egg showed the highest fresh weight of Cordyceps militaris. While the culture media comprising of Sinlek rice with either pupa or glucose, yeast and peptone showed a trend of higher Cordycepin content than the other treatments. By-products of the Cordyceps militaris that was cultured by Sinlek rice and
pupa was used to produce the ready to eat jelly product in sachet packaging. The results showed that the panelists had overall acceptance of the jelly products at the slightly like level and the jelly products had natural colour of the Cordyceps militaris juice extract.
