The research aimed to produce the bacterial cellulose film (BDF) and to study the physical properties of the film obtained. The experiment was carried out by using bacterial cellulose (BC) from Acetobecter xylinum with rambutan juice as a carbon source. The BC was dried and mixed with sodium alginate as a co-film former at the ratioof 1:1, glycerol and sorbitol as a plasticizer at 0.2, 0.4 and 0.6%. The film was prepared by pouring 20g of the mixture into a petri dish with a diameter of 9 cm. It was them dried in a hot oven at a temperature of 50C for 36 hours. After that, the film was tested for ease of peeling, water vapor transmission rate (WVTR) and grease and oil resistance (GOR).